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Tubi HPLC

HPLC Tubing

HPLC tubing, also known as capillary or chromatographic tubing, is a critical component of HPLC systems. It functions as a conduit for the mobile phase and sample, enabling their flow throughout the chromatographic setup.

HPLC tubing is characterized by its small diameter, typically ranging from 1 mm to 5 mm or smaller. The narrow diameter allows for efficient liquid flow and reduces system volume, minimizing sample dilution and enhancing separation resolution.

The length of HPLC tubing varies based on system complexity and requirements, spanning from a few centimetres to several meters. Longer tubing lengths can increase separation efficiency and provide more time for interaction between the sample and stationary phase.

The inner surface of HPLC tubing must be smooth and inert to minimize interactions with the sample. A smooth surface reduces the potential for sample adsorption or carryover between runs, ensuring accurate and reproducible results.

HPLC tubing is connected to various system components, including injectors, columns, and detectors, using fittings or connectors. Secure and leak-free connections are vital to maintain system integrity and prevent sample loss or contamination.

The choice of HPLC tubing depends on factors such as analyte properties, required flow rate, and compatibility with the mobile phase and solvents. Tubing materials must exhibit chemical resistance, low adsorption characteristics, and mechanical stability to withstand system pressures and temperatures.

HPLC tubing is available in various materials, each with its own considerations and advantages in terms of chemical compatibility, thermal stability, and mechanical strength.

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  • PEEK GF30

    PEEK GF30 refers to a grade of PEEK material that is reinforced with 30% glass fiber.

    PEEK GF30 sheets or plates have the same base PEEK polymer as PEEK 450G but with the added enhancement of glass fiber reinforcement. The glass fibers enhance the mechanical properties of PEEK, providing increased strength, stiffness, and dimensional stability.

    PEEK GF30 sheets retain the other desirable properties of PEEK, such as high-temperature resistance, chemical resistance, and low friction coefficient.

    These sheets are commonly used in applications where improved mechanical performance, wear resistance, and strength are required, such as automotive components, aerospace parts, electrical connectors, and industrial applications.

  • PEEK 450g

    PEEK 450G refers to a specific grade of PEEK material. The "450G" designation signifies a standard, unfilled grade of PEEK manufactured by Victrex. PEEK 450G sheets or plates are composed solely of PEEK polymer without any reinforcement materials like glass fibers or carbon fibers. These sheets offer the inherent properties of PEEK, including high temperature resistance, excellent mechanical strength, chemical resistance, low friction coefficient, and dimensional stability.

    PEEK 450G sheets are typically available in various thicknesses and sizes to suit different application requirements.

    They find applications in industries such as aerospace, automotive, electronics, medical, and more, where the outstanding properties of PEEK are beneficial.

  • We use these two types of PEEK due to the enhanced qualities they have over standard PEEK meaning that our tubing is more effective in the HPLC application. These Benefits are:

  • Enhanced Purity

    Victrex 450g PEEK undergoes stringent manufacturing processes and quality controls, resulting in higher purity levels compared to generic PEEK. This enhanced purity reduces the risk of impurities and contaminants, ensuring better performance and minimizing interference with samples or processes.

  • Improved Mechanical Properties

    Victrex 450g PEEK offers enhanced mechanical strength, stiffness, and toughness compared to standard PEEK. These improvements can result in better resistance to stress, cracking, and vibration, ensuring reliable performance in demanding applications.

  • Optimized Chemical Resistance

    Victrex 450g PEEK exhibits superior chemical resistance compared to generic PEEK. It may provide better resistance to a wide range of solvents, acids, bases, and other aggressive chemicals, ensuring long-term stability and reliability in harsh chemical environments.

  • Enhanced Dimensional Stability

    Victrex 450g PEEK offers improved dimensional stability, maintaining its shape and dimensions even under high temperatures or prolonged use. This enhanced stability contributes to tighter tolerances, better sealing, and improved overall performance in critical applications.

  • Tailored Formulation

    Victrex 450g PEEK has specific modifications or formulation adjustments to enhance certain properties based on targeted applications. These modifications could be related to thermal stability, wear resistance, electrical conductivity, or other performance parameters, resulting in a more optimized material for specific use cases.

What Is PEEK And Why Do We Use It In Our HPLC Tubing

PEEK (polyether ether ketone) is a high-performance thermoplastic polymer with excellent mechanical, thermal, and chemical properties. It is widely used in various industries, including HPLC, due to its desirable characteristics.

PEEK is employed in HPLC tubing primarily because of its chemical compatibility and inertness. As HPLC involves the separation and analysis of various compounds, it is essential to use tubing materials that do not react or interact with the sample components. PEEK exhibits exceptional resistance to a wide range of solvents, acids, bases, and other chemicals commonly used in HPLC. Its inert nature ensures that it does not introduce unwanted interactions or contaminations that could affect the accuracy or reliability of the analysis.

Additionally, PEEK tubing offers high mechanical strength and stability, allowing it to withstand the pressures and temperatures experienced during HPLC analysis. It maintains its dimensional integrity under the operational conditions of the system, ensuring consistent flow and reliable results.

PEEK also possesses good thermal stability, enabling its use in HPLC systems that operate at elevated temperatures. This property is particularly beneficial in applications such as reversed-phase chromatography, where higher temperatures can enhance separation efficiency. Furthermore, PEEK is a biocompatible material, making it suitable for use in HPLC applications involving biological samples or biomolecules.

We use two different types of PEEK for our tubing which are:

I tubi HPLC realizzati in polietereterchetone (PEEK) sono una scelta popolare nei sistemi di cromatografia liquida ad alte prestazioni (HPLC) grazie alla sua eccellente resistenza chimica, elevata resistenza meccanica e basse proprietà estraibili. Il PEEK è un polimero termoplastico che mostra un'eccezionale stabilità in condizioni di alta pressione e alta temperatura, rendendolo adatto per applicazioni HPLC impegnative. Qui descriverò e spiegherò le caratteristiche e i vantaggi dei tubi PEEK nei sistemi HPLC:

  1. Resistenza chimica: i tubi in PEEK sono altamente resistenti a un'ampia gamma di solventi organici, acidi, basi e sali. Può resistere a fasi mobili aggressive e mantenere la propria integrità senza degradazione o lisciviazione di contaminanti. Questa resistenza chimica garantisce la purezza e l'affidabilità del sistema HPLC, prevenendo qualsiasi interazione indesiderata tra il tubo e il campione o la fase mobile.

  2. Elevata resistenza meccanica: i tubi in PEEK possiedono eccellenti proprietà meccaniche, tra cui elevata resistenza alla trazione e resistenza allo stress e agli urti. Può resistere alle alte pressioni tipicamente riscontrate nei sistemi HPLC senza distorsioni o guasti. La robustezza dei tubi in PEEK garantisce prestazioni affidabili e costanti, anche in condizioni operative impegnative.

  3. Basso volume morto: il tubo in PEEK ha un diametro interno piccolo, con conseguente basso volume morto. Il volume morto si riferisce al volume di liquido trattenuto all'interno dei tubi e dei raccordi, che può causare un allargamento della banda e influenzare la risoluzione cromatografica. Il basso volume morto dei tubi in PEEK riduce al minimo la dispersione del campione e consente picchi più nitidi, migliorando le prestazioni cromatografiche del sistema HPLC.

  4. Inerzia: il PEEK è un materiale altamente inerte, il che significa che non reagisce né adsorbe analiti o componenti della fase mobile. Questa inerzia è fondamentale per un'analisi HPLC accurata e riproducibile, poiché impedisce qualsiasi interazione indesiderata o perdita di analiti durante il processo di separazione. I tubi in PEEK garantiscono un assorbimento minimo del campione, garantendo l'integrità e l'affidabilità dell'analisi quantitativa e qualitativa.

  5. Biocompatibilità: il PEEK è biocompatibile, il che lo rende adatto per applicazioni HPLC che comportano l'analisi di campioni biologici, come peptidi, proteine ​​e composti farmaceutici. Non introduce contaminanti né interferisce con i componenti del campione, preservando l'integrità e l'affidabilità dei test bioanalitici.

  6. Stabilità della temperatura: il tubo in PEEK presenta un'eccellente stabilità termica, che gli consente di resistere ai gradienti di temperatura elevati incontrati nei sistemi HPLC. Mantiene la stabilità dimensionale e le proprietà meccaniche in un ampio intervallo di temperature, consentendo un funzionamento efficiente sia in condizioni HPLC analitiche che preparative.

  7. Facilità d'uso: i tubi in PEEK sono leggeri e flessibili, il che li rende facili da maneggiare, tagliare e collegare all'interno di un sistema HPLC. Può essere facilmente tagliato alla lunghezza desiderata e collegato facilmente a vari componenti HPLC, come valvole di iniezione, rilevatori e porte di ingresso/uscita della colonna. La flessibilità dei tubi in PEEK facilita l'installazione e la manutenzione dei sistemi HPLC.

  8. Compatibilità: i tubi in PEEK sono compatibili con un'ampia gamma di componenti del sistema HPLC, inclusi raccordi, connettori e rilevatori. Può essere facilmente integrato nelle configurazioni HPLC esistenti senza la necessità di modifiche o adattatori estesi.

In sintesi, i tubi in PEEK sono un materiale altamente adatto per le applicazioni HPLC grazie alla sua resistenza chimica, elevata resistenza meccanica, basso volume morto, inerzia, biocompatibilità, stabilità alla temperatura, facilità d'uso e compatibilità con i componenti del sistema HPLC. Garantisce prestazioni affidabili e costanti, consentendo separazioni accurate e riproducibili in varie applicazioni HPLC analitiche e preparative.